The L3MBTL1 TR-FRET Assay Kit is designed to measure the inhibition of L3MBTL1 binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, L3MBTL1, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

Q9Y468

L3MBTL1 assay kit, HTRF

DMSO >1%, strong acids or bases, ionic detergents, high salt

COMPONENTS

See data sheet for detailed protocol.

Stable at least 6 months from date of receipt, when stored as directed

Useful for the study of MBT domain binding assays, screening inhibitors, and selectivity profiling

Min, J., et al., Nat Struct Mol Biol.2008 Jan,15(1):114

Avoid freeze/thaw cycles

Methyl-lysine Reader

Assay Kit