This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience’s cell lines, including:

• TGF/SMAD Signaling Pathway SBE Reporter – HEK293 Cell Line #60653

• AP-1 Reporter – HEK293 Recombinant Cell Line (JNK signaling pathway) #60405

Sterile filtered (0.2 µm) and Mycoplasma-free

All components are low endotoxin

For detailed cellular assay protocol, see cell line data sheet.

MEM medium (Hyclone #SH30024.01) supplemented with 0.5% FBS (Thermo Fisher, #26140079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01).

Each lot is tested for growth of recombinant cells. FBS is ISIA-certified and sourced from the United States of America.

Upon receipt, the medium should be stored in the dark at 4°C. Avoid repeated freeze/thaw cycles. Do not exceed 37°C. When stored in the dark at 4°C, the product is stable for six months after first thaw.

Cell Signaling Pathway

Media