Human CD226 antigen also known as DNAX accessory molecule 1 (DNAM-1), GenBank Accession No. NM_006566, a.a. 19-247 fused to Fc region of human IgG1, this protein is enzymatically biotinylated using AviTag^TM technology and expressed in a HEK293 cell expression system. MW = 54.7 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Q15762

DNAX accessory molecule 1 (DNAM-1)

CD226, biotin binding to CD155, His-tag was confirmed according to the protocol for BPS Cat. 72052. Briefly, 6 ng of CD226-biotin was mixed with varying amounts of CD155, His-tag in CD226 assay buffer at a final volume of 10 µl and allowed to incubate for 1 hr at room temp. Nickle acceptor beads were added for 30 min, followed by Strep-conjugated acceptor beads for 30 min and reading alpha-counts.

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

At least 6 months at –80°C.

Useful for studying protein binding and for screening small molecules and antibodies.

1. Bottino, C., et al., J. Exp. Med. 2003; 198(4): 557-567.
2. Hafler, J., et al., Genes Immun. 2009; 10: 5-10.

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Avoid freeze/thaw cycles. Protein may be diluted to 100 μg/ml in PBS + glycerol and stored at - 80°C.

Immunotherapy/Cell Surface Receptor

Recombinant Protein