The CD40:CD40L[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD40:CD40L signaling. This kit comes in a convenient 96-well format, with biotin-labeled CD40, purified CD40L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled CD40 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD40L is coated on a 96-well plate. Next, CD40 is incubated with CD40L on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

P29965

T cell antigen Gp39, TNF related activation protein, TRAP, tumor necrosis factor ligand superfamily member 5, TNFSF5, CD154, CD40, CD40 Molecule, activity assay kit, Strep HRP, streptavidin

This kit comes in a convenient 96-well format, with biotin-labeled CD40, purified CD40L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.

See assay kit data sheet for detailed protocol

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

This kit is useful for screening for inhibitors of CD40L binding to CD40.

Avoid freeze/thaw cycles.

Immunotherapy

Assay Kit