The GITR:GITRL TR-FRET Assay is designed to measure the inhibition of GITR binding to GITRL in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated GITR, GITRL, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.

Q7TS55

Glucocorticoid-induced TNFR-Related Protein, Tumor Necrosis Factor Receptor Superfamily member 18, TNFRSF18, , HTRF

See assay kit data sheet for detailed protocol

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for screening small molecular inhibitors for drug discovery and HTS applications.

1. Lechner, M.G., et al. Immunotherapy. 2011 Nov; 3(11): 1317–1340.

2. Ko, K., et al., J. Exp. Med. 2005; 202(7): 885-891.

Avoid freeze/thaw cycles

Immunotherapy

Assay Kit