This cell line expresses a surface human GITR (glucocorticoid-induced TNFR family related gene; TNFRSF18; CD357) and an NF-ĸB luciferase reporter construct that are stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-ĸB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.

NFKB, GITR, NF-KB

Each vial contains ~2 x 106 cells in 1 ml of 10% DMSO in FBS.

Jurkat

This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Cat. #LT07-518) was used as a positive control.

Thaw Medium 2 (BPS Cat. #60184): RPMI1640 medium (Life Technologies, #A10491-01) supplemented with 10% FBS (Life Technologies, #26140-079), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01).

Growth Medium 2C (BPS Cat. #79592): Thaw Medium 2 (BPS Cat. #60184), plus 100 µg/ml Hygromycin (Thermo Fisher, #10687010) and 1 mg/ml G418 (Thermo Fisher, #11811031).

Store in liquid nitrogen immediately upon receipt.

The GITR NF-ĸB-luciferase/Jurkat cell line is suitable for monitoring the activity of NF-kB signaling in response to GITR ligand (GITRL).

Esparza, E.M. and Arch, R.H. (2005) Glucocorticoid-Induced TNF Receptor, a Costimulatory Receptor on Naïve and Activated T Cells, Uses TNF Receptor-Associated Factor 2 in a Novel Fashion as an Inhibitor of NF-kB Activation. J Immunology. 174: 7875-7882.

License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Immunotherapy

Cell Line