| 产品名称 | TIGIT:CD112 Homogeneous Assay Kit |
| 产品货号 | 72030 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 384 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: Human T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is a receptor
that is expressed on the surface of human T cells and NK cells that binds to CD155 and CD112
on the surface of dendritic cells. Binding of TIGIT with CD155 or CD112 results in inhibition of T
cell and NK cell activation. Antibodies and other agents that inhibit this signaling pathway have
been shown to increase the immune response, especially in the case of certain cancers.
Description: The TIGIT:CD112 Homogeneous Assay Kit is designed to measure the
inhibition of human T-cell immunoreceptor with Ig and ITIM domains (TIGIT) binding to CD112
(PVRL2/Nectin-2). The TIGIT:CD112 Homogeneous Assay Kit comes in a convenient
AlphaLISA® format with purified biotinylated TIGIT, His-tagged CD112, and assay buffer to
perform a total of 384 reactions. With this kit, only three simple steps on a microtiter plate are
required. First, a sample containing TIGIT and an inhibitor of choice is incubated with the
CD112 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by reading
the Alpha-counts.
Synonym(s): T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9 (VSIG9), and V-set and transmembrane domaincontaining protein 3 (VSTM3), PVRL2, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen
Contraindications: Only limited amounts of DMSO can be included, as it has been shown to disrupt TIGIT:CD112 interaction. Avoid green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.
Format:
Instructions for use: See assay kit datasheet for detailed protocol.
Storage / Stability: At least one year from date of receipt when stored as directed.
Application(s): Useful for screening for inhibitors of TIGIT binding to CD112
Reference(s): 1. Yu, X., et al., Nat. Immunol. 2009; 10(1): 48-57. Notes: AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Immunotherapy/Cell Surface Receptor
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
|
| 产品资料 |
