| 产品名称 | TIGIT:CD155 Homogeneous Assay Kit |
| 产品货号 | 72029 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 384 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: Human T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is a receptor
that is expressed on the surface of human T cells and NK cells that binds to CD155 and CD112
on the surface of dendritic cells. Binding of TIGIT with CD155 or CD112 results in inhibition of T
cell and NK cell activation. Antibodies and other agents that inhibit this signaling pathway have
been shown to increase the immune response, especially in the case of certain cancers.
Description: The TIGIT:CD155 Homogeneous Assay Kit is designed to measure the
inhibition of TIGIT binding to CD155. The TIGIT:CD155 Homogeneous Assay Kit comes in a
convenient AlphaLISA® format with purified biotinylated TIGIT, His-tagged CD155, and assay
buffer to perform a total of 384 reactions. With this kit, only three simple steps on a microtiter
plate are required. First, a sample containing TIGIT and an inhibitor of choice is incubated with
the CD155 for 60 minutes. Next, acceptor beads are added, then donor beads, followed by
reading the Alpha-counts.
Synonym(s): T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, VSIG9, V-set and transmembrane domain-containing protein 3, VSTM3, PVR, NECL-5, CD155-His, AlphaLISA, Alphascreen, Alpha Lisa, Alpha screen
Contraindications: Only limited amounts of DMSO can be included, as it has been shown
to disrupt TIGIT:CD155 interaction. Avoid green and blue dyes that absorb light in the
AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the
potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+,
Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due
to the presence of excess biotin and iron in this medium. MEM, which lacks these components,
does not affect AlphaScreen assays.
Format:
Instructions for use: See assay datasheet for detailed protocol.
Storage / Stability: At least one year from date of receipt when stored as directed.
Application(s): Useful for screening for inhibitors of TIGIT binding to CD155
Reference(s): 1. Yu, X., et al., Nat. Immunol. 2009; 10(1): 48-57. Notes: AlphaScreen® and AlphaLISA® are registered trademarks of PerkinElmer, Inc.
Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Immunotherapy/Cell Surface Receptor
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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