| 产品名称 | SHP-1 (PTPN6) |
| 产品货号 | 30021 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 20 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 93 kDa
Genbank #: NM_080549
Tag(s): N-terminal GST-tag
a.a: 1-577
Background: PTPN6 gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The noncatalytic N-terminus of this PTP can interact with MAP kinases and negatively regulates ERK2 and p38 MAP-kinases activity. The PTPN6 was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway.
Description: Protein tyrosine phosphatase SHP-1, also known as PTPN6 (GenBank accession number NM_080549), a.a. 1-577, with N-terminal GST-tag, MW=93 kDa, expressed in an E. coli expression system.
UniProt P25440
Synonym(s): SHP1; SHP-1; HCP; HCPH; HPTP1C; PTP-1C; SHP-1L; SH-PTP1, SHP
Specific Activity: 1 U/µg
Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Assay Conditions: Enzyme reaction (50
μl) containing 20 mM HEPES, pH 7.2, 100
mM NaCl, 2 mM EDTA, 10 mM DTT, 1
mM pNPP and PTPase was was
monitored at 415 nm continuously with
readings at 1 minute intervals for 20
minutes.
Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
Format: Aqueous buffer solution
Storage / Stability: At least 6 months at –80˚C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Application(s): Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Reference(s): 1. N.E. Kilgore et al., J. Immunol. 2003, 170:4891-4895.
2. J.V. Ravetch & L.L. Lanier Science 2000, 290: 84-9. 3. C. Duchesne et al. J. Biol. Chem 2003, 278:14274-14283. Warning(s): Avoid freeze/thaw cycles
Scientific Category: PTP/Non-rec.
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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