Human B- and T-lymphocyte attenuator (BTLA), also known as CD272, with C-terminal Avi-TagTM fused to the Fc-region of Human IgG1. GenBank Accession No. NM_181780, a.a. 31-150, expressed in a HEK293 cell expression system. MW = 42 kDa (monomer). This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation.

CD272

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

Aqueous buffer solution

At least 6 months at –80°C

1. Flynn R, et al., PLoS One. 2013 Oct 29;8(10):77991.

2. Uchiyama M, et al., Transplantaion. 2014 Feb 15;97(3):301-9.

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Immunotherapy

Protein