The PRDM9 Chemiluminescent Assay Kit is designed to measure PRDM9 activity for screening and profiling applications, using purified PRDM9. The PRDM9 Chemiluminescent Assay Kit comes in a convenient format, with 8-well strips pre-coated with histone H3 peptide substrate, an antibody against methylated lysine on Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and enough purified PRDM9 enzyme for 100 enzyme reactions. The key to the PRDM9 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K36 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

• TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween-20)
• 0.5 M DTT (dithiothreitol) in anhydrous DMSO
• Luminometer or fluorescent microplate reader capable of reading chemiluminescence
• Adjustable micropipettor and sterile tips
• Rotating or rocker platform

 Powers NR, et al. PLoS Genet. 2016 Jun 30; 12(6):e1006146.