Lactacystin is a cell-permeable, potent and selective proteasome inhibitor that binds irreversibly to the catalytic beta-subunit of the 20S proteasome inhibiting its chymotrypsin-like and trypsin-like activities. Also known to inhibit NF-kappaB activation (IC50 = 10 μM).

N–Acetyl–S–[(3S,4R)–3–hydroxy–2–[(1S)–1–hydroxy–2–methylpropyl]–4–methyl–5–oxo–D–prolyl]–L–cysteine

>99% by TLC.

Soluble in Water (10 mg/ml) or DMSO (20 mg/ml)

White powder

Useful for inhibition of the 20S proteasome.

1. Fenteany, et al. Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin. Science 1995, 268:726.
2. Kozlowski, L. et al. Lactacystin inhibits cathepsin A activity in melanoma cell lines. Tumour Biol. 2001, 22:211.
3. Pasquini, L.A. et al. Lactacystin, a specific inhibitor of the proteasome, induces apoptosis and activates caspase-3 in cultured cerebellar granule cells. J. Neurosci Res. 2000, 59:601.

Protect from light. Protect from moisture. Avoid freeze/thaw cycles.

Ubiquitin Enzymes

Inhibitors/Activators