UCHL1, also known as Ubiquitin Carboxy-terminal Hydrolase L1 or PGP9.5 (GenBank Accession No. NM_004181), a.a. 2-224(end), with N-terminal His-tag, MW=23.4 kDa, expressed in E. coli.

P09936

UCHL1, Ubiquitin Carboxy-terminal Hydrolase L1, PGP9.5, Ubiquitin C-terminal Hydrolase L1,

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and UCHL1. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation of 340 nm, and emission at 460 nm.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Gong, B., et al., (2006). Cell 126 (4): 775– 788.
2. Healy DG, et al., (2005). Cell Tissue Res. 318 (1): 189–94.

Avoid freeze/thaw cycles

Ubiquitin Hydrolase

Recombinant Protein