Human Ataxin-3 (ATX3 or ATXN3), also known as Josephin (JOS), Machado-Joseph disease protein 1 (MJD1) or Spinocerebellar ataxia type 3 (SCA3), GenBank Accession No. NM_004993, a.a. 2-362(end) with an N-terminal His-tag, MW=42.1 kDa, expressed in E. coli.

P54252

Ataxin-3, ATX3, ATXN3, Josephin, JOS, Machado-Joseph disease protein 1, MJD1, Spinocerebellar ataxia type 3, SCA3

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and Ataxin-3 (0-0.4 ng/µL) in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. do Carmo Costa, M., et al. (2010). PLoS One 5(7):e11728.
2. Jung, J., et al. (2009). Hum Mol Genet. 18(24):4843-52.
3. Macedo-Ribeiro, S., et al. (2009). PLoS One 4(6): e5834.

Avoid freeze/thaw cycles

Ubiquitins: Deubiquitinase (DUB)

Recombinant Protein