| 产品名称 | MuRF1, SUMO-His-tags - Ligases (E3) - Ubiquitination |
| 产品货号 | 81052 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 25 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 40 kDa
Genbank #: NP_115977
Tag(s): N-terminal SUMO-His-tags
a.a: full length
Background: MuRF1 (Muscle-specific RING-finger protein 1) is an E3 (ubiquitin protein ligase) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E2 enzymes. MuRF1 is also known as TRIM63 (tripartite motif containing 63) containing a RING-finger/B-box/coiled-coil tripartite fold and is a member of the RING-domain E3 family. MuRF1 has been implicated as a component of atrophy-associated accelerated proteolysis. It is upregulated in a number of different models of atrophy and interacts with numerous myofibrillar proteins.
Description: Human MuRF1 recombinant protein (Genbank Accession No. NP_115977), full length with N-terminal SUMO- and His-tags, expressed in E. coli.
Synonym(s): TRIM63, tripartite motif-containing 63
Purity: ≥90% by SDS-PAGE
Biological Activity: Typical enzyme concentration of 100 nM - 5 mM is used for in vitro conjugation, depending on conditions.
Formulation: 50 mM Tris Buffer, pH 7.6, 150 mM NaCl, 10% glycerol
Format: Aqueous buffer solution
Storage / Stability: Stable for ≥1 year at –80°C. Application(s): Useful as an E3 ligase in ubiquitin-conjugating reactions and to analyze upregulation/downregulation of E3 enzymes.
Reference(s): 1. Bodine, S.C., et al., Identification of ubiquitin ligases required for skeletal muscle atrophy. Science , 2001. 294:1704-8.
2. Centner, T., et al., Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain. J Mol Biol. 2001. 306:717-26. 3. Kedar, V., et al., Muscle-specific RING finger 1 is a bona fide ubiquitin ligase that degrades cardiac troponin I. Proc Natl Acad Sci USA, 2004. 101:18135-40. 4. Mrosek, M., et al., Molecular determinants for the recruitment of the ubiquitin-ligase MuRF-1 onto M-line titin. FASEB J. 2007. 21:1383-92. Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Ubiquitination (Ubiquitin Ligase (E3))
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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