产品名称 | CUL3/Rbx1, GST-tag - Ligases (E3) - Ubiquitination |
产品货号 | 80409 |
产品价格 | 现货询价,电话:010-67529703 |
产品规格 | 20 µg |
产品品牌 | bpsbioscience |
产品概述 | |
产品详情 |
Species: Human
Host Species: Sf9 Insect Cell
MW: CUL3: 117 kDa; Rbx1: 12 kDa
Genbank #: CUL3: NM_003590; Rbx1: NM_014248
Tag(s): N-terminal GST-tag
a.a: CUL3: 2-768(end); Rnx1: 1-108(end)
Description: Complex of human cullin-3 (CUL3) with human RING box protein 1 (Rbx1), also known as E3 ubiquitin-protein ligase RBX1. CUL3 (a.a. 2-768(end) with N-terminal GSTtag, 117 kDa) is co-expressed with Rbx1 (a.a. 1-108(end), 12 kDa) in a baculovirus infected Sf9 cell expression system. GenBank Accession Nos. are NM_003590 (CUL3) and NM_014248 (Rbx1).
UniProt CUL3: Q13618; Rbx1: P62877
Synonym(s): Cullin-3, RING box protein 1, RING box protein 1, E3 ubiquitin protein ligase 1
Formulation: 40 mM Tris-HCl, pH 8.0,
110 mM NaCl, 2.2 mM KCl, 0.04%
Tween-20, 20% glycerol, and 1.6 mM
glutathione.
Format: Aqueous buffer solution
Storage / Stability: At least 6 months at -80°C
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Kaspar, J. et al., J. Biol. Chem. 2010; 285: 21349-21358 2. Kaspar, J. et al., J. Cell. Sci. 2012; 125: 1027-1038. Warning(s): Avoid freeze/thaw cycles. Storing diluted
enzyme is not recommended, if
necessary, use carrier protein (BSA 0.1
– 0.5%).
Scientific Category: Ubiquitin E3 ligase
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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产品资料 |