| 产品名称 | CBL-B GST Tag |
| 产品货号 | 80415 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 100 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 72 kDa
Genbank #: NM_170662
Tag(s): N-terminal GST-Tag
a.a: 39 – 426
Background: CBL-B, a RING finger E3-ubiquitin ligase, acts as an intracellular checkpoint and a regulator of cytotoxic lymphocytes and NK cells. CBL-B binds E2-ubiqutin as well as target proteins such as TAM receptor TYRO3, forcing further degradation of TYRO3. CBL-B modulates innate immune responses and plays a key role in host defense to pathogens and anti-tumor immunity. Targeting CBL-B represents a potential therapeutic strategy for the management of human immune-related disorders such as autoimmune diseases, infections, tumors, and allergic airway inflammation. CBL-B has emerged as a new target of interest in the field of immunotherapy due to its ability to suppress cancer immune-evasion. CBL-B initiates anti-cancer immune responses in NK cells in both cytotoxic and effector T cells, promoting T-cell and NK cell activation.
Description: Human Casitas B-lineage
lymphoma proto-oncogene b (CBL-B), also
known as E3 ubiquitin-protein ligase CBL-B,
and RING finger protein 56, GenBank
Accession No. NM_170662, a.a. 39 – 426 with
N-terminal GST-Tag expressed in an E. coli
expression system. MW=72 kDa.
Synonym(s): E3 ubiquitin-protein ligase CBL-B, RING finger protein 56, CBL, CBLB
Assay Conditions: 10 uL of reaction mixture contains 50 mM Tris, pH 7.4, 5 mM MgCl2, 0.01% Tween, 1 mM DTT, 0.3 µM Ub[Biotin], 100 nM Ube1, 1 mM ATP, 2 µM of UBCH5b, and various amount of CBL-B. The reaction was initiated with the addition of ATP, and incubated at 30° C for 3 hours. Tb labeled anti-GST antibody donor and XL665 labeled acceptor was added and incubated at room temperature for an hour.
Formulation: 40 mM HEPES pH 7.4, 120 mM NaCl, 20% glycerol.
Storage / Stability: At least 6 months at –80˚C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Application(s): Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Reference(s): 1. Jeon, M.S., et al., Immunity. 2004; 21(2): 167-177.
2. Chiang, Y.J. et al., Nature. 2000; 403: 216-220. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Ubiquitin E3 ligase
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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