UEV1A (ubiquitin-conjugating enzyme E2 variant 1), also known as UBE2V1 or CROC-1 (GenBank Accession No. NM_021988), a.a. 2-170(end), with N-terminal FLAG-tag, MW=20 kDa, expressed in E. coli.

Q13404

UEV1A, UBE2V1, CROC-1, ubiquitin-conjugating enzyme E2 variant 1

50 μl reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, UBE1 (0.5 μg), UEV1A or other E2, Biotin-Ubiquitin (0.5 μg) and Smurf2 (2 μg) in 50 mM Tris, pH 7.4) for 30 min at 37°C. Add to Streptavidin-coated plates for 30 min at 37°C. Incubate with anti-Smurf2, then HRP-labeled secondary antibody, and chemiluminescent detection.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml Flag peptide, 10% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at -80°C.

Useful in conjunction with E1 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1.   Thomson TM, et al. (1998). FEBS Lett. 423 
     (1): 49–52.  
2.  Hau  DD,  et  al.  (2006).  Biochemistry  45 
     (32): 9866–77. 
3.  Ma  L,  et  al.  (1998).  Oncogene  17  (10): 
     1321–6. 

Avoid freeze/thaw cycles

Ubiquitin Conjugating Enzyme (E2)

Recombinant Protein