UbcH7, also known as UBE2L3 (GenBank Accession No. NM_003347), a.a. 1-154 (end), with C-terminal His-tag, MW = 18.7 kDa, expressed Sf9 cells via a baculovirus expression system.

P68036

UbcH7, UBE2L3, ubiquitin-conjugating enzyme E2L3

50 μl reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, UBE1 (0.5 μg), UbcH7, Biotin-Ubiquitin (0.5 μg) and Smurf2 (1.5 μg) in 50 mM Tris, pH 7.4) for 30 min at 37°C. Add to Streptavidin-coated plates for 30 min at 37°C. Incubate with anti-Smurf2, then HRP-labeled secondary antibody, and chemiluminescent detection.

50 mM Tris, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, 50% glycerol and 1 mM DTT

Aqueous buffer solution

>6 months at -80°C.

Useful in conjunction with E1 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Avoid freeze/thaw cycles

Ubiquitin Conjugating Enzyme (E2)

Recombinant Protein