| 产品名称 | NEDD8 E1, His-tag - Activating Enzyme (E1) - Ubiquitination |
| 产品货号 | 81087 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 50 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 61 kDa (APPBP1), 52 kDa (UBA3)
Tag(s): His-tag (APPBP1), no tag (Uba3)
Background: The NEDD8 activating E1 enzyme is a heterodimer composed of APPBP1 and UBA3 subunits. The APPBP1/UBA3 enzyme has homology to the N- and C-terminal halves of the ubiquitin E1 enzyme, respectively. The UBA3 subunit contains the catalytic center and activates NEDD8 in an ATP-dependent reaction by forming a high-energy thiolester intermediate. The activated NEDD8 is subsequently transferred to the UbcH12 E2 enzyme, and is then conjugated to specific substrates in the presence of the appropriate E3 ligases.
Description: Human NEDD8 E1 recombinant protein complex, with His-tagged APPBP1 and untagged Uba3, co-expressed in E. coli.
UniProt Q8TBC4
Synonym(s): Nedd8, APPBP1/Uba3
Purity: ≥80%
Biological Activity: 1–100 nM is used for the in vitro conjugation
Formulation: 50 mM Tris Buffer, pH 8.0, 150 mM NaCl, 10% glycerol
Format: Aqueous buffer solution
Storage / Stability: Store at or below –80°C. Stable as supplied for up to 1 year when stored dessicated at –80°C. Application(s): Useful for NEDD8 conjugation reactions and NEDDylation pathway studies
Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Ubiquitination (Ubiquitin Activating Enzyme (E1))
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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