Human A20, also known as tumor necrosis factor- induced protein 3 (TNFAIP3) or OTU domain-containing protein 7C (OTUD7C), GenBank Accession No. NM_006290, amino-acids 2-790(end) with an N-terminal FLAG-tag and C-terminal His-tag, MW=91 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

Q9UHC1

tumor necrosis factor-induced protein 3, TNFAIP3, OTU domain-containing protein 7C, OTUD7C, A20

0.766 pmol/min/µg

Reaction was performed in 50 mM Tris pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and various amount of A20 in a volume of 50 μL. Reaction was incubated at 37°C for 30 min and fluorescent signal was measured at excitation of 360 nm, and emission at 460 nm.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween- 20, 3 mM DTT, and 20% glycerol

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7.
2. Tavares, R.M., et al. (2010). Immunity 33(2):181-9.
3. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41

Avoid freeze/thaw cycles

Ubiquitins: Deubiquitinase (DUB)

Recombinant Protein