Recombinant human serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PP1A), also known as PPP1CA and PPP1A, GenBank Accession No. NM_002708, full-length with an N-terminal GST tag and expressed in a baculovirus-infected Sf9 insect cell expression system. MW = 62 kDa.

P62136

serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PPP1CA, PPP1A

228 pmol/min/µg

Assay was done using BIOMOL GREEN Reagent. Briefly, 5 μl of varying amounts of PP1A was mixed with 10 μl of buffer containing 0.2% 2- mercaptoethanol and 65 ng/μl BSA and 5 μl of 1 μg/μl Thr-phosphopeptide synthetic substrate (KRT(p)IRR). Reaction was incubated at 37°C for 15 min. before adding 100 μl of BIOMOLE Green Reagent and incubating at room temp. for 30 min. Absorbance was read at 650 nm.

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol

Aqueous buffer solution.

At least 6 months at –80°C.

Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Cohen, P. et al., J. Biol. Chem. 1989; 264 21435-21438.
2. Genoux, D. et al., Nature. 2002; 418: 970- 975.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Protein phosphatase - Serine

Recombinant Protein