| 产品名称 | SHP-2, His-Tag - Non-Receptor - Protein Phosphatase(s) |
| 产品货号 | 79018 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 20 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 70 kDa
Genbank #: NM_002834
Tag(s): N-terminal His-tag
a.a: 2-593
Background: Mammalian PTPases can be subdivided into 1 of 2 broad categories: transmembrane receptor PTPases and intracellular PTPases. PTPN11 is one of the 2 closely related mammalian intracellular PTPases whose sequences encode 2 tandem SRC homology 2 (SH2) domains that are located at the amino-terminal side of a single PTPase catalytic domain. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.
Description: Human recombinant SHP-2, also known as PTPN11 (protein tyrosine phosphatase N11), (a.a. 2-593 (end) (full length), with N-terminal His-tag, Genbank accession number NM_002834, MW=70 kDa, expressed in an E. coli expression system.
Synonym(s): SHP2, CFC, NS1, BPTP3, PTP2C, PTP-1D, SH-PTP2, SH-PTP3, MGC14433, SHP
Specific Activity: >50 pmol/min/µg Assay Conditions: 50mM HEPES, pH7.4, 100mM NaCl, 2mM EDTA, 3mM DTT, 5mM PNPP substrate, 30 min at 30°C. Absorbance is measured at 405nM.
Formulation: 40mM Tris pH8.0, 110mM NaCl, 2.2mM KCl, 0.04% Tween-20 and 20% glycerol.
Format: Aqueous buffer solution
Storage / Stability: At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. B.G. Neel et al. Trends Biochem. Sci. (2003) 28: 284
2. J.V. Ravetch & L.L. Lanier Science (2000) 290: 84 Warning(s): Avoid freeze/thaw cycles
Scientific Category: PTP/Non-rec.
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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