| 产品名称 | MEG-1 (PTPN4), GST-tag - Non-Receptor - Protein Phosphatase(s) |
| 产品货号 | 30015 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 20 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 60.3 kDa
Genbank #: NM_002830
Tag(s): N-terminal GST-tag
a.a: 637–926
Background: PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This protein contains a C-terminal PTP domain and an N-terminal domain homologous to the band 4.1 superfamily of cytoskeletal-associated proteins. This PTP acts at junctions between the membrane and the cytoskeleton and has been shown to interact with glutamate receptor delta 2 and epsilon subunits. It also is thought to play a role in signalling downstream of the glutamate receptors through tyrosine dephosphorylation.
Description: MEG-1 protein tyrosine phosphatase (PTP-MEG-1), Genbank Accession No. NM_002830, a.a. 637-926 (catalytic domain) with N-terminal GST-tag, MW=60.3 kDa, expressed in an E. coli expression system.
Synonym(s): MEG1, MEG-1, PTPN4, Megakaryocyte Phosphatase
Specific Activity: 4.6 U/µg
Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Assay Conditions: The specific activity was determined using pNPP. Assay conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 20 mM pNPP, and 20 ng/µl enzyme for 5 minutes at 30°C.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format: Aqueous buffer solution
Storage / Stability: >12 months at -80°C. Application(s): Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Reference(s): 1. K. Hironaka, et al., J. Biol.Chem. 2000, 275: 16167-16173
2. M. Gu, et al. Proc. Natl. Acad. Sci. 1996, 93: 12980-12985 Warning(s): Avoid freeze/thaw cycles
Scientific Category: PTP/Non-rec.
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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