| 产品名称 | PTPμ (PTPRM), GST-tag - Protein Phosphatase(s) |
| 产品货号 | 30053 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 20 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 56.2 kDa
Genbank #: NM_001105244
Tag(s): N-terminal GST-tag
a.a: 896–1175
Background: PTPRM is a member of the protein tyrosine phosphatase family and can participate in a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPRM has been shown to mediate cell-cell aggregation through the interaction with another molecule of PTPRM on an adjacent cell. PTPRM can interact with scaffolding protein RACK1/GNB2L1 and this interaction may be necessary for downstream signaling in response to cell-cell adhesion. PTPRM has been shown to be expressed in human pulmonary vascular endothelia where it directly binds to VE-cadherin and regulates both the tyrosine phosphorylation state of VE-cadherin and barrier integrity.
Description: PTPμ (mu) (protein tyrosine
phosphatase mu, also known as RPTPµ),
catalytic domain, (a.a. 896-1175), (GenBank
Accession No. NM_001105244) with
N-terminal GST tag, MW=56.2 kDa,
expressed in an E. coli expression system.
Specific Activity: 60 U/µg
Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and 2 ng/µl PTP-mu. Assay was done at room temperature for 5 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1.
Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione
Format: Aqueous buffer solution
Storage / Stability: >12 months at -80°C. Application(s): Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Reference(s): 1. S. Brady-Kalnay, et al., J. Cell Biol 1995, 130: 977.
2. T. Mourton, et al., J. Biol. Chem. 2002, 276:14896. Warning(s): Avoid freeze/thaw cycles
Scientific Category: PTP/Receptor
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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