| 产品名称 | PTPβ, GST-tag - Protein Phosphatase(s) |
| 产品货号 | 30042 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 20 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 63.7 kDa
Genbank #: X54131
Tag(s): N-terminal GST-tag
a.a: 1675–1996
Background: Plays an important role in blood vessel remodeling and angiogenesis. Not necessary for the initial formation of blood vessels, but is essential for their maintenance and remodeling. Can induce dephosphorylation of TEK/TIE2, CDH5/VE-cadherin and KDR/VEGFR-2. Regulates angiopoietin-TIE2 signaling in endothelial cells. Acts as a negative
regulator of TIE2, and controls TIE2 driven endothelial cell proliferation, which in turn affects blood vessel remodeling during embryonic development and determines blood vessel size during perinatal growth. Essential for the maintenance of endothelial cell contact integrity and for the adhesive function of VE-cadherin in endothelial cells.
Description: Protein tyrosine phosphatase PTPβ (beta), also known as PTPRB (GenBank accession No. X54131), a.a. 1675-1996 (catalytic domain) with N-terminal GST-tag, MW=63.7 kDa, expressed in an E. coli expression system.
Synonym(s): PTPB, PTPRB, VEPTP, PTPbeta, RPTbeta, PTPβ
Specific Activity: 57 nmole/min/µg
Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and PTPβ. Assay was done at 30&Deg;C for 5 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1.
Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione
Format: Aqueous buffer solution
Storage / Stability: >12 months at -80°C. Application(s): Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Reference(s): 1. I. K. Lund et al., J Biol Chem. 2004, 279: 24226-35.
2. G.H. Peters et al., Biochimie. 2003, 85: 527-34. Warning(s): Avoid freeze/thaw cycles
Scientific Category: PTP/Receptor
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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