Protein phosphorylation is one of the most important post-translational modification processes. Phosphorylation is reversibly regulated by Protein Kinases (PKs) and Protein Phosphatases (PTPs). PTP1B (PTPN1) is known to catalyze dephosphorylation of insulin receptor kinases and plays a critical role in insulin signaling. The PTP1B (1-321) Colorimetric Assay Kit is designed to measure PTP1B activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. The PTP1B assay kit comes in a convenient 96-well format, with purified PTP1B enzyme, colorimetric substrate, and PTP assay buffer for 100 enzyme reactions. The PTP1B (1-321) Colorimetric Assay Kit is optimized for both continuous and end-point assay formats.

PTPN1, Protein tyrosine phosphatase 1B, ptp

The PTP1B (1-321) Colorimetric Assay Kit comes in a convenient 96-well format, with purified PTP1B enzyme, colorimetric substrate, and PTP assay buffer for 100 enzyme reactions.

See assay kit data sheet for detailed protocol

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

1. Barrett, W.C., et al., Biochem. 1999; 38: 6699-6705.

Application Reference(s): 
1. Protein-Tyrosine Phosphatase-1B Mediates Sleep Fragmentation-Induced Insulin Resistance and Visceral Adipose Tissue Inflammation in Mice (2017)

Avoid freeze/thaw cycles.

Protein Phosphatase

Assay Kit