产品名称 Fluorogenic PTP1B (Catalytic Region) Assay Kit - Protein Phosphatase(s)
产品货号 79764
产品价格 现货询价,电话:010-67529703
产品规格 96 reactions
产品品牌 bpsbioscience
产品概述
产品详情
Description:

Protein phosphorylation is one of the most important post-translational modification processes. Phosphorylation is reversibly regulated by Protein Kinases (PKs) and Protein Phosphatases (PTPs). PTP1B (PTPN1) is known to catalyze dephosphorylation of insulin receptor kinases and plays a critical role in insulin signaling. The Fluorogenic PTP1B (Catalytic Region) Assay Kit is designed to inhibitors of the catalytic region of PTP1B in a homogeneous assay with no time-consuming washing steps. Note: To identify inhibitors of the regulatory region of PTP1B, please use our Fluorogenic PTP1B (Full Length) Assay Kit, #79766).

Materials Required But Not Provided:
Fluorescent microplate reader capable of reading ex/em=360nm/460nm
Synonym(s):
PTPN1, Protein tyrosine phosphatase 1B, ptp
Supplied As:
he PTP1B assay kit comes in a convenient 96-well format, with purified PTP1B enzyme, fluorogenic substrate, and PTP assay buffer for 96 enzyme reactions.
Format:

Catalog #

Component

Amount

Storage

30010

Recombinant Human PTP1B (1-321)*

≥1 µg

-80°C

Avoid freeze/ thaw cycles!

 

0.5 mM PTP1B Substrate

50 µl

-80°C

79716

5X PTP Assay Buffer

20 ml

-20°C

 

0.5 M DTT

20 µl

-20°C

79685

Black, low binding black microtiter plate

1

Room Temperature

 

*Additional material has been provided for ease of retrieval.

Instructions for use:
See assay protocol for detailed instructions.
Storage / Stability:
One year from date of receipt when stored as directed.
Application(s):
Great for studying enzyme kinetics and HTS applications.
Reference(s):

Gee, K.R., et al., Anal Biochem, 1999 Aug 15; 273(1):41-8.

Brown-Shimer, S., et al., 1992 Jan 15; 52(2):478-82.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Protein Phosphatase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

79764.pdf
产品资料