Human cathepsin S (GenBank Accession No. NM_004079.3), CD33 signal peptide(a.a. 1-16) + cathepsin S (a.a. 17-331), with C-terminal His-tag, MW=37 kDa, expressed in FreeStyle 293-F cells.

P25774

CTSS

≥ 700 pmole/min/µg

The enzymes were diluted in 25 mM MES buffer pH5, 0.1 M NaCl, 5 mM DTT, and activated by low pH at room temperature for 2 hours. After addition of 10 μM substrate (Z-L-R-AMC) a kinetic reaction was followed for 30 minutes at room temperature and the fluorescence intensity was measured at exc360/em460.

45 mM Tris7HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 3 mM DTT, and 10% glycerol

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Seo H.R. et al., Int. J. Cancer 124 (8), 1794-1801 (2009).
2. Lutzner N. and Kalbacher H. J. Biol. Chem. 283 (52), 36185-36194 (2008).

Avoid freeze/thaw cycles

Protease

Recombinant Protein