Human cathepsin B (GenBank Accession No. NM_001908), CD33 signal peptide (a.a. 1-16) + CathepsinB (a.a. 18-339), with C-terminal His-tag, MW=43 kDa, expressed in FreeStyle 293-F cells.

P07858

Amyloid Precursor Protein Secretase, CTSB, APP Secretase

≥ 4048 pmol/min/µg

25 mM MES, pH 5.0, 10 µM substrate (Z-Leu-Arg-AMC), 10 min, room temperature. Prior to assay enzyme was activated with preincubation at 10 µg/ml in 25 mM MES, pH 5.0, 5 mM DTT for 15 min at RT.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Tu, C. et al., Cancer Res. 2008 Nov 15;68(22):9147-56.
2. Hwang, JH. et al., Cancer Lett. 2009 Jan 18;273(2):266-72.

Avoid freeze/thaw cycles

Protease

Recombinant Protein