The Fluorogenic Prolyl OligoPeptidase (POP) Assay Kit is a complete assay system designed to measure activity of the purified POP enzyme. The Fluorogenic POP Activity Kit eliminates the dealing with radioactive materials and chromatography in traditional assays. Purified human recombinant POP is included in the kit as a positive control. Using this kit, only one simple step, in which the fluorometric substrate is incubated with purified POP, is needed to analyze the POP activity level. The resulting fluorescent product can then be easily measured with a microtiter-plate fluorimeter.

P48147

Prolyl Oligopeptidase, POP, PREP, Prolyl Endopeptidase

Supplied in a convenient 96-well format, with purified POP enzyme, substrate, and assay buffer for 100 enzymereactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt

At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

1. Myohanen T.T. et al., Neurochem. Res. 32 (8), 1365-1374 (2007).
2. Garcia-Horsman J.A. et al., Scand. J. Gastroenterol. 42 (5), 562-571 (2007).

Application Reference(s):
1. New compounds identified through in silico approaches reduce the α-synuclein expression by inhibiting prolyl oligopeptidase in vitro (2017)

The AMC standard is included so the researcher can quatitatively determine the specific activity of the enzyme using the AMC standard as a measure of how much AMC substrate was cleaved to release free AMC.

Avoid freeze/thaw cycles

Protease

Assay Kit