产品名称 | Cathepsin L, His-tag - Protease(s) |
产品货号 | 80005 |
产品价格 | 现货询价,电话:010-67529703 |
产品规格 | 10 µg |
产品品牌 | bpsbioscience |
产品概述 | |
产品详情 |
Species: Human
Host Species: FreeStyle 293-F cells
MW: 37 kDa
Genbank #: NM_001912
Tag(s): C-terminal His-tag
a.a: 18–333
Description: Human cathepsin L (GenBank Accession No. NM_001912), a.a. 18-333, with C-terminal His-tag, MW=37 kDa, expressed in FreeStyle 293-F cells.
UniProt P07711
Synonym(s): CTSL, MEP
Specific Activity: ≥ 6000 pmol/min/µg
Assay Conditions: The enzyme reactions were conducted in 50mM MES buffer pH5.0, 100mM NaCl, 5mM DTT and 20μM substrate (Z-Leu-Arg-AMC) 30 minutes at room temperature. The fluorescence intensity was measured at exc360/em460.
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 250 mM Imidazole, and 20% glycerol
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for activation of DPP1 (Cathepsin C). Suitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Jean D. et al., Biochem. J. 413 (1), 125-134 (2008).
2. Abboud-Jarrous G. et al., J. Biol. Chem. 283 (26), 18167-18176 (2008). Warning(s): Avoid freeze/thaw cycles
Scientific Category: Protease
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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产品资料 |