Bace1 (β-secretase 1) is an aspartic protease that is involved in the processing of the Amyloid precursor protein (APP). Cleavage of APP by BACE1 followed by γ-secretase results in β-amyloid peptide production, which ultimately leads toxic Aβ accumulation. In Alzheimer’s disease (AD), it has been widely accepted that Aβ aggregation plays a critical role in AD pathogenesis, suggesting that BACE1 could be a potential target to treat AD. The BACE1 FRET Assay Kit is designed to measure BACE1 activity for screening and profiling applications based on fluorescence resonance energy transfer (FRET) using a labeled peptide substrate (below).

beta-site amyloid precursor protein cleaving enzyme 1, ASP2

The BACE1 FRET Assay Kit comes in a convenient 96-well format, with enough purified recombinant human BACE1 enzyme, BACE1 FRET peptide substrate, and assay buffer for 100 enzyme reactions.

COMPONENTS: 

See assay kit data sheet for detailed protocol.

At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Ghosh, A.K., et al. J. Neurochemistry 120(suppl. 1):71-83 (2012)

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Microplate reader capable of reading fluorescence Adjustable micropipettor and sterile tips

Avoid freeze/thaw cycles.

Neuroscience

Assay Kit