Human activated protein C, also known as PROC, APC, or active coagulation factor XIV, (GenBank Accession No. NM_000312), a.a. 1 – 461 (end) with C-terminal His-tag, expressed in an HEK293 cell expression system. Note: Protein is secreted and converted to the active form with disulfide link (light chain a.a. 43-199, heavy chain a.a. 212-461). The signal peptide (a.a. 1-32), propeptide (a.a. 33-42), and activation peptide (a.a. 200-211) are cleaved. MW = ~60 kDa, ~42 kDa, ~20 kDa under reducing conditions.

P04070

PROC, APC, activated protein C, active coagulation factor XIV

≥ 95 pmol/min/µg

50 mM Tris-HCl, pH 8.5, 150 mM NaCl, 10 mM CaCl₂, 0.01% Brij-35, 100 µM substrate, and various amounts of Activated Protein C. Incubate for 10 minutes at room temperature. Fluorescence intensity is measured at exc380/em460.

50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, and 250 mM imidazole

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors,and selectivity profiling.

1. Wagener, G., et al., Transplant. Proc. 2012,44(5):1336-1340.
2. Luo, L., et al., Mediators Inflamm. 2012,2012:485279.

Avoid freeze/thaw cycles

Protease

Recombinant Protein