The TNKS2 Histone Ribosylation Assay Kit (Antibody Detection) is designed to measure Tankyrase-2 activity for screening and profiling applications. The key to the TNKS2 Histone Ribosylation Assay Kit (Antibody Detection) is a highly specific antibody that recognizes poly (ADP-ribose)-modified histone. After coating the plate with the histone mixture, only three simple steps are required. First, NAD+ is incubated with a sample containing assay buffer and enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Tankyrase2, TNKS2, poly (ADP-ribose) polymerase 5, poly(ADP-ribosyl)transferase, PARP5b, PARP5c

The TNKS2 Histone Ribosylation Assay Kit (Antibody Detection) comes in a convenient format, with 8-well strips, histone mixture substrate, antibody against poly (ADP-ribose) modified histone, the secondary HRP-labeled antibody, NAD+, PARP assay buffer, an

DMSO >1%, strong acids or bases, ionic detergents, high salt

CatalogNumber	Reagent	Amount	Storage
80515	TNKS2	2 µg	-80°C	(Avoid freeze/thaw cycles!)
52029	5× histone mixture	1 ml	-80°C
	NAD+	250 µl	-80°C
80602	10x PARP Assay Buffer	1 ml	-20°C
52140K	Primary antibody 11	12.5 µl	-80°C
52130H	Secondary HRP-labeled antibody 1	10 µl	-80°C
79743	Blocking buffer 3	2 x 40 ml	+4°C
79670	ELISA ECL substrate (2 components)	6 ml each	+4°C
	8-well strip plate module	1 plate (12 x 8-well strips)	+4°C

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.

This kit is also suitable for use with cell extracts, but nonspecific ribosylation activity may be detected.

Avoid freeze/thaw cycles.

PARP

Assay Kit