The Tankyrase-1 Histone Ribosylation Assay Kit (Antibody Detection) is designed to measure Tankyrase-1 activity for screening and profiling applications. The key to the Tankyrase-1 Histone Ribosylation Assay Kit (Antibody Detection) is a highly specific antibody that recognizes poly (ADP-ribose)-modified histone. With this kit, only three simple steps on a microtiter plate are required for Tankyrase-1 reaction. First, NAD+ is incubated with a sample containing assay buffer and enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Tankyrase1, TNKS1, poly (ADP-ribose) polymerase 5a, poly(ADP-ribosyl)transferase 5a, PARP5a

Kit comes in a convenient format, with a 384-well plate precoated with histone mixture, antibody against poly (ADP-ribose) modified histone, the secondary HRP-labeled antibody, NAD+, TNKS assay buffer, and purified TNKS1 enzyme for 384 enzyme reactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.

This kit is also suitable for use with cell extracts, but nonspecific ribosylation activity may be detected.

Avoid freeze/thaw cycles.

PARP

Assay Kit