The PARP1 Colorimetric Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones. The key to the PARP1 Colorimetric Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the colorimetric HRP substrate to produce color that can then be measured using a UV/Vis spectrophotometer microplate reader.

poly (ADP-ribose) polymerase 1, poly(ADP-ribosyl)transferase, ADPRT, PARP1, PARP-1

The PARP1 assay kit comes in a convenient 96-well format, with purified PARP1 enzyme, histone mixture, activated DNA, and PARP1 assay buffer for 100 enzyme reactions.

See assay kit data sheet for detailed protocol

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

1.Brown, J.A., Marala, R.B. 2002. J. Pharmacol. Toxicol. Methods 47:137-41.

Application Reference(s):
1. Olaparib hydroxamic acid derivatives as dual PARP and HDAC inhibitors for cancer therapy (2017)

Avoid freeze/thaw cycles.

PARP

Assay Kits