世联博研公司现货供应bpsbioscience品牌订货号为80569的PARP1 Chemiluminescent Assay Kit (384-well) - Assay Kits

产品名称

PARP1 Chemiluminescent Assay Kit (384-well) - Assay Kits - PARP(s)

产品货号

80569

产品价格

现货询价，电话：010-67529703

产品规格

384 reactions

产品品牌

bpsbioscience

产品概述

产品详情

Species: Human

Description:

The PARP1 Chemiluminescent Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NADdependent addition of poly (ADP-ribose) to histones. The key to the PARP1 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Synonym(s):

poly (ADP-ribose) polymerase 1, poly(ADP-ribosyl)transferase, ADPRT, PARP1, PARP-1

Supplied As:

The PARP1 assay kit comes in a convenient 384-well format, with purified PARP1 enzyme, histone mixture, activated DNA, and PARP1 assay buffer for 400 enzyme reactions.

Contraindications:

DMSO >1%, strong acids or bases, ionic detergents, high salt

Format:

Catalog #	Reagent	Amount	Storage
80501	PARP1	2 x 5 µg	-80°C	*Avoid multiple freeze/ thaw cycles!*
52029	5x histone mixture	2 x 1 ml	-80°C
80601	10x Assay Mixture Containing Biotinylated Substrate	2 x 300 µl	-80°C
80602	10x PARP assay buffer	2 x 1 ml	-20°C
79743	Blocking buffer 3	2 x 25 ml	+4°C
80605	Activated DNA (5X)	2 x 500 µl	-80°C
80611	Streptavidin-HRP	2 x 100 µl	+4°C
79670	ELISA ECL Substrate A (translucent bottle)	2 x 6 ml	+4°C
79670	ELISA ECL Substrate B (brown bottle)	2 x 6 ml	+4°C
	384-well plate	1	Room Temp.

Instructions for use:

See assay kit datasheet for detailed protocol.

Storage / Stability:

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Reference(s):

Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41.

Application Reference(s):
1. Neuroprotective effects of a novel poly (ADP-ribose) polymerase-1 inhibitor, JPI-289, in hypoxic rat cortical neurons (2017)
2. Dichamanetin inhibits cancer cell growth by affecting ROS-related signaling components through mitochondrial-mediated apoptosis (2013)
3. Structural basis and SAR for G007-LK, a lead stage 1,2,4-triazole based specific tankyrase 1/2 inhibitor (2013)

Notes:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
1x PBS buffer PBST buffer (1x PBS, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform

Warning(s):

Avoid freeze/thaw cycles.

Scientific Category:

PARP

Product Type:

Assay Kit

Data shown is lot-specific. Contact us for specific information on other lots.

Qty:

Quality Assurance

Product Datasheets

80569.pdf

产品资料