Human PDE3B (GenBank Accession No. NM_000922), a.a. 592-end, with N-terminal GST-tag, MW = 86 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Q14432

PDE3B, phosphodiesterase 3B

≥ 1270 pmol/min/µg

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Assayed using BPS's PDE3B Assay Kit, Cat. # 60331. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE3B at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

40 mM Tris, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.04% Tween-20, 20% glycerol

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Raymond, D.R., et al., Cell. Signal. 19 (12), 2507-2518 (2007).
2. Palmer, D., et al., J. Biol. Chem. 282 (13), 9411-9419 (2007).

Application References:
1. Utility of Adenosine Monophosphate Detection System for Monitoring the Activities of Diverse Enzyme Reactions (2017)
2. Identification and characterization of a potent and biologically-active PDE4/7 inhibitor via fission yeast-based assays (2017)
3. A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013)
4. Cyclic phosphatidic acid stimulates cAMP production and inhibits growth in human colon cancer cells (2013)

Avoid freeze/thaw cycles.

PDE

Recombinant Protein