Dog PDE3A, also known as Phosphodiesterase 3A, CGMP-Inhibited 3',5'-Cyclic Phosphodiesterase A, and CGI-PDE A, GenBank Accession No. XP_005637137, a.a. 343-998 (end), with C-terminal FLAG-tag, expressed in a HEK293 expression system, MW = 75 KDa.

Phosphodiesterase 3A, cGMP-Inhibited 3',5'-Cyclic Phosphodiesterase A, and CGI-PDE A

Aqueous buffer solution.

10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 μM cAMP, 0.02% TritonX, and serial dilutions of PDE3A at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Absorbance was measured at 620 nm.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3mM DTT and 20% glycerol.

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Hung, S.H., et al. J. Biol. Chem. 281 (39), 29236-29244 (2006)
2. Hambleton, R., et al. J. Biol. Chem. 280 (47), 39168-39174 (2005)

Avoid multiple freeze/thaw cycles.

PDE

Protein