Human PDE2A1 (GenBank Accession No. NM_002599), a.a. 2-941, with N-terminal FLAG-tag, MW=106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

O00408

PDE2A, phosphodiesterase 2A

≥ 7577 pmol/min/μg

10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 μM cAMP, and PDE2A1 at 37ºC for 20 min. Quantified by 5-nucleotidase cleaving the 5-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 10% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Seybold, J., et al., Blood 105 (9), 3569- 3576 (2005).
2. Martinez, S.E., et al., Proc. Natl. Acad. Sci. U.S.A. 99 (20), 13260-13265 (2002).

Avoid freeze/thaw cycles.

PDE

Recombinant Protein