Murine PDE2A, variant 2 (GenBank Accession No. NM_001008548), a.a 2-935, with N-terminal GST tag, MW=132 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

≥20,000 pmole/min/µg. One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C. Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE2A at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 1.6 mM glutathione, 20% glycerol

At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Sadhu, K., et al., JHC 47(7), 889-894 (1999).

2. Iffland, A., et al., Biochemistry 44 (23), 8312-8325 (2005).

PDE

Enzyme