| 产品名称 | PDE1C TR-FRET Assay Kit - Assay Kits - Phosphodiesterase(s) |
| 产品货号 | 60705 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 96 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE1C is a calmodulin-dependent PDE that is expressed principally in human myocardium. The assay is based on the generation of FAM-labeled nucleotide monophosphates by the phosphodiesterase. These phosphate groups bind to terbium-labeled nanoparticles, resulting in energy transfer from the terbium to the FAM, which emits a fluorescent signal at 520 nm. The change in fluorescent intensity can be easily measured using a fluorescence plate reader.
Description: The PDE1C TR-FRET Assay Kit is designed for identification of inhibitors of PDE1C using TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) technology. Using this kit, only two simple steps on a microtiter plate are required for the PDE1C activity assay. First, the fluorescent-labeled cAMP is incubated with a sample containing PDE1C for 1 hour. Second, a binding agent and a terbium donor are added to the reaction mix and incubated for 30 minutes. Then, fluorescence intensity can be measured using a fluorescence reader.
Synonym(s): inhibitor screening, FRET assay kit, PDE1C, HTRF
Supplied As: The PDE1C TR-FRET Assay Kit comes in a convenient 96-well format, with purified PDE1C enzyme, fluorescently labeled PDE1C substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Format:  Instructions for use: See assay kit data sheet for detailed protocol.
Storage / Stability: At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival. Application(s): Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Reference(s): Vandeput. F., et al. J. Biol. Chem. 2007; 282(45): 32749-32757.
Warning(s): Avoid freeze/thaw cycles
Scientific Category: PDE
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
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