Human Histone 2B, GenBank Accession No. NM_003528, a.a. 2-126(end) with N-terminal His-tag, MW = 14.6 kDa, expressed in an E. coli expression system.

Q16778

H2B/c,H2BFC,HIST1H2BL,Histone H2B

50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 10% glycerol, 1 mM PMSF and 1 mM DTT.

Aqueous buffer solution

>6 months at –80°C.

Useful as a substrate for histone methyltransferase and acetyltransferase assays. Ideal for screening small molecular inhibitors of histone modifying enzymes for drug discovery and HTS applications.

1.   Osley,  M.A.,  Brief  Funct.  Genomic Proteomic. 5(3):179-89 (2006). 
2.  Wyrick,  J.J.  and  Parra,  M.A.,  Biochim Biophys Acta. 1789(1):37-44 (2009).   
3.   Emre, N.C.  and Berger, S.L., Cold Spring Harb  Symp  Quant  Biol.  69:  289-99 (2004).

Application Reference(s):
Differential mode of antimicrobial actions of arginine-rich and lysine-rich histones against Gram-positive Staphylococcus aureus (2013)

Avoid freeze/thaw cycles

Methyltransferase/Substrate

Recombinant Protein