Human DNMT3a (GenBank Accession No. NM_175629), full length with N-terminal GST-tag, MW=128 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

Q9Y6K1

DNA

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 18 mM glutathione, and 10% glycerol.

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Shikauchi, Y. et al., Mol. Cell. Biol. 29 (7):1944-1958 (2009).
2. Zhao, Q. et al., Nat. Struct. Mol. Biol. 16 (3):304-311 (2009).

Application Reference(s):
DNA 5-methylcytosine demethylation activities of the mammalian DNA methyltransferases (2013)

Avoid freeze/thaw cycles.

Methyltransferase

Recombinant Protein