The EZH2 Direct Activity Chemiluminescent Assay Kit is designed to measure activity of the EZH2 complex (EZH2/EED/SUZ12/RbAp48/AEBP) for screening and profiling applications. The key to the EZH2 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH

Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, reagents, and purified EZH2 complex for 100 enzyme reactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Dillon S.C., et al. 2005. Genome Biology 6:227.

Avoid freeze/thaw cycles.

Methyltransferase

Assay Kit