| 产品名称 | EZH2 (Y641N) TR-FRET Assay Kit - Assay Kits - Methyltransferase(s) |
| 产品货号 | 52078 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 384 reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
Description: The EZH2 (Y641N) TR-FRET Assay Kit is designed to measure activity of the
mutant EZH2 complex [EZH2 (Y641N)/EED/SUZ12/RbAp48/AEBP] in a homogeneous 384
reaction format. This FRET-based assay requires no time-consuming washing steps, making it
especially suitable for high throughput screening applications. The key to the EZH2 (Y641N) Assay Kit is a
highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three
simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine
is incubated with a sample containing assay buffer and methyltransferase
enzyme for 4 hours. Next, antibody to methylated H3K27 is added. Finally, dye-labeled acceptor
is added followed by fluorescence detection.
Synonym(s): HTRF
Supplied As: The EZH2 (Y641N) TR-FRET Assay Kit comes in a convenient format, with histone H3 peptide substrate, a Tb-labeled antibody against methylated K27 residue of Histone H3, S-adenosylmethionine, methyltransferase assay buffer, TR-FRET detection buffer, dye-lab
Format:
Instructions for use: See datasheet for detailed protocol.
Storage / Stability: At least 6 months from date of receipt when stored as directed.
Reference(s): 1. Dillon, S.C., et al. Genome Biology 2005; 6:227 Notes: MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Adjustable micropipettor and sterile tips This mutation is often cited in the scientific literature as Y641N in variant 3 (GenBank Accession No. NM_001203247). Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Methyltransferases
Product Type: Assay Kits
Data shown is lot-specific. Contact us for specific information on other lots.
|
| 产品资料 |
