The EZH1 Chemiluminescent Assay Kit is designed to measure activity of the EZH1 complex (EZH1/EED/SUZ12/RbAp48/AEBP) for screening and profiling purposes. The key to the EZH1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRPlabeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Q92800

KMT6B, EZH

The EZH1 Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, an antibody against methylated K27 residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay

COMPONENTS:

See product datasheet for detailed protocol.

One year from date of receipt when stored as directed.

Great for studying enzyme kinetics and HTS applications.

1. Dillon SC, et al. Genome Biol. 2005; 6:227.
2. Morin, RD., et al. Nat. Genet. 2010, 42(2):181.

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
TBST buffer (1x TBS, pH 8.0, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Rotating or rocker platform

Avoid freeze/thaw cycles.

Methyltransferase

Assay Kit