The DOT1L Chemiluminescent Assay Kit is designed to measure DOT1L activity for screening and profiling applications. The key to the DOT1L Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K79 residue of H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for two hours. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

KMT4, KIAA1814

The DOT1L Chemiluminescent Assay Kit comes in a convenient format, with a 96-well plate, DOT1L substrate, primary antibody against methylated lysine residue of Histone H3, secondary HRP-labeled antibody, Sadenosylmethionine, methyltransferase assay buffer

COMPONENTS:

See product datasheet for detailed protocol.

One year from date of receipt when stored as directed.

Great for studying enzyme kinetics and HTS applications.

1. Min, J., et al. Cell 2003; 112:711-723.
2. Daigle, S.R., et al. Cancer Cell 2011, 20:53-65.

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
TBST buffer (1x Tris-buffered saline, pH 8.0, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Rotating or rocker platform

Avoid freeze/thaw cycles.

Methyltransferase

Assay Kit