The DNMT3A Direct Activity Assay Kit is designed to measure DNMT3A activity for screening and profiling applications. The key to the DNMT3A Direct Activity Assay Kit is a highly specific antibody that recognizes 5-methylcytosine of the substrate. With this kit, only three simple steps on a microtiter plate are required for detection of DNMT3A activity. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT3A/3L for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

DNA

Kit comes in a convenient format, with a 96-well plate precoated with DNMT substrate, an antibody against 5-methylcytosine, a secondary HRP-labeled antibody, S-adenosylmethionine, DNMT assay buffer, and purified DNMT3A/3L complex for 100 enzyme reactions.

DMSO >1%, strong acids or bases, ionic detergents, high salt

COMPONENTS:

See assay kit data sheet for detailed protocol.

12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Useful for studying enzyme kinetics and HTS applications.

Svedruzic, Z.M. Curr Med Chem. 2008; 15(1) :92-106.

Avoid freeze/thaw cycles.

Methyltransferase

Assay Kit